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platelet derived growth factor aa  (R&D Systems)


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    Structured Review

    R&D Systems platelet derived growth factor aa
    Platelet Derived Growth Factor Aa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 162 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+pdgf+aa/Recombinant+Human+PDGF-AA+Protein%2C+CF/bio_rxiv__64898__2026__03__29__715075-61-11-20
    Average 95 stars, based on 162 article reviews
    platelet derived growth factor aa - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: Overexpression of CD47 is associated with brain overgrowth in 16p11.2 deletion syndrome
    Article Snippet: Subsequently, cells were detached using a Corning cell lifter (Sigma-Aldrich) and transferred to ultra-low-attachment plates for the formation of spheres. .. OPC differentiation was induced in the OPC induction media containing DMEM/F-12, 1× MEM non-essential amino acids (NEAA) solution (Life Technologies), 1× GlutaMAX (Life Technologies), 2-mercaptoethanol 1× (Life Technologies), Penicillin/Streptomycin (PenStrep; Life Technologies), 1× N-2 supplement (Life Technologies), B27 Supplement without VitA (Life Technologies), 10 ng/ml Recombinant human PDGF-AA, CF (R&D Systems), 10 ng/ml Recombinant human IGF-I, CF (R&D Systems), 5 ng/ml Recombinant human HGF (R&D Systems), 10 ng/ml Neurotrophin 3 (NT3; EMD Millipore), 25 μg/ml Insulin solution, human (Sigma-Aldrich), 100 ng/ml Biotin (Sigma-Aldrich), 1 μM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP; Sigma-Aldrich) and 60 ng/ml 3,3,5-Triiodo-l-thyronine (T3; Sigma- Aldrich). .. OPC differentiation was induced in the OPC induction media containing DMEM/F-12, 1× MEM non-essential amino acids (NEAA) solution (Life Technologies), 1× GlutaMAX (Life Technologies), 2-mercaptoethanol 1× (Life Technologies), Penicillin/Streptomycin (PenStrep; Life Technologies), 1× N-2 supplement (Life Technologies), B27 Supplement without VitA (Life Technologies), 10 ng/ml Recombinant human PDGF-AA, CF (R&D Systems), 10 ng/ml Recombinant human IGF-I, CF (R&D Systems), 5 ng/ml Recombinant human HGF (R&D Systems), 10 ng/ml Neurotrophin 3 (NT3; EMD Millipore), 25 μg/ml Insulin solution, human (Sigma-Aldrich), 100 ng/ml Biotin (Sigma-Aldrich), 1 μM N6,2′-O-Dibutyryladenosine 3′,5′-cyclic monophosphate sodium salt (cAMP; Sigma-Aldrich) and 60 ng/ml 3,3,5-Triiodo-l-thyronine (T3; Sigma- Aldrich).

    Article Title: Platelet-Derived Growth Factor Receptor α Contributes to Human Hepatic Stellate Cell Proliferation and Migration
    Article Snippet: Cells were cultured/expanded in Techno Plastic Product–treated T75 tissue culture flasks (Sigma-Aldrich, St. Louis, MO). .. Recombinant human PDGF-AA (R&D Systems, Minneapolis, MN) or PDGF-BB (Sigma-Aldrich) and TGF-β1 (R&D Systems) were diluted with serum-free media and exposed to cells for the indicated time periods after two washes with sterile, cold PBS. ..

    Article Title: Neuropilin-1 and platelet-derived growth factor receptors cooperatively regulate intermediate filaments and mesenchymal cell migration during alveolar septation
    Article Snippet: For proximity ligation assay, goat polyclonal anti-NRP1 (catalog no. AF566) was obtained from R&D Systems; rabbit polyclonal anti-PDGFRα (catalog no. sc-431) from Santa Cruz Biotechnology; rabbit polyclonal anti-phosphorylated (Y410) p130Cas (BCAR-1; catalog no. ab55263) from Abcam; and Duolink in situ orange starter kit, goat/rabbit (catalog no. DUO92106-1KT) from Sigma Aldrich. .. Another reagent, recombinant human PDGF-AA (catalog no. 221-AA), was obtained from R&D Systems. ..

    Article Title: Protective effects of 4-aminopyridine in experimental optic neuritis and multiple sclerosis.
    Article Snippet: .. OPCs were maintained in proliferation-supporting high-glucose DMEM-based Sato medium (Thermo Fisher Scientific) supplemented with 10 ng/ml recombinant human bFGF (PeproTech) and 10 ng/ml recombinant human PDGF-AA (R&D Systems). .. Alternatively, differentiation was initiated by Sato medium depleted of growth factors and supplemented with 0.5% foetal calf serum (PAA Laboratories).

    Article Title: Rescuing the negative impact of human endogenous retrovirus envelope protein on oligodendroglial differentiation and myelination.
    Article Snippet: Department of Neurology, Medical Faculty, Heinrich-Heine-University, Düsseldorf, Germany GeNeuro, Chemin du Pré-Fleuri 3, Geneva, Switzerland Correspondence Patrick Küry, PhD, Neuroregeneration laboratory, Department of Neurology, Heinrich-Heine-University, Moorenstrasse 5, D-40225 Düsseldorf, Germany.. Email: kuery@uni-duesseldorf.de Funding information ARSEP/AFM; Deutsche Forschungsgemeinschaft, Grant/Award Number: KU1934/2_1KU1934/5_1; James and Elisabeth Cloppenburg, Peek & Cloppenburg Düsseldorf Stiftung; Research commission of the medical faculty of the Heinrich-Heine-University; Walter and Ilse Rose Foundation Abstract Remyelination in the adult CNS depends on activation, differentiation, and functional integration of resident oligodendroglial precursor cells (OPCs) and constitutes the only spontaneous neuroregenerative process able to compensate for functional deficits upon loss of oligodendrocytes and myelin sheaths as it is observed in multiple sclerosis.. The proteins encoded by p57kip2and by human endogenous retrovirus type W (pHERV-W) envelope genes were previously identified as negative regulators of OPC maturation.

    Article Title: Data on G-quadruplex topology, and binding ability of G-quadruplex forming sequences found in the promoter region of biomarker proteins and those relations to the presence of nuclear localization signal in the proteins
    Article Snippet: All non-labelled and FITC-labelled DNA oligonucleotides were purchased from Eurofins Genomics (Tokyo, Japan) with HPLC purification and stored in TE buffer (10 mM Tris–HCl, 0.1 mM EDTA; pH8.0) at the concentration of 100 μM. .. VEGFA (VEGF165 and VEGF121) and recombinant human PDGF-AA, PDGF-BB and PLGF were purchased from R&D Systems (Minneapolis, MN, USA). .. Recombinant human RB1 and the intracellular domain of recombinant human c-KIT (corresponding to amino acids 544–976) were purchased from Abcam (Cambridge, UK).

    Sterility:

    Article Title: Platelet-Derived Growth Factor Receptor α Contributes to Human Hepatic Stellate Cell Proliferation and Migration
    Article Snippet: Cells were cultured/expanded in Techno Plastic Product–treated T75 tissue culture flasks (Sigma-Aldrich, St. Louis, MO). .. Recombinant human PDGF-AA (R&D Systems, Minneapolis, MN) or PDGF-BB (Sigma-Aldrich) and TGF-β1 (R&D Systems) were diluted with serum-free media and exposed to cells for the indicated time periods after two washes with sterile, cold PBS. ..



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    Proteintech pdgf aa
    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    R&D Systems pdgf aa protein
    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated <t>with</t> <t>PDGF-AA</t> or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.
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    (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Journal: bioRxiv

    Article Title: INPP5E interactome reveals novel connections to growth factor signaling

    doi: 10.64898/2026.02.13.705725

    Figure Lengend Snippet: (a) Inpp5e +/‒ and Inpp5e ‒/‒ MEFs were stimulated with PDGF-AA or FBS for the indicated times and cell lysates analyzed by Western blot with the indicated antibodies. Actin was used as loading control. (b-c) Quantitations of normalized pAKT/AKT ratios from n=4 (S473) or n=3 (T308) independent experiments, including the one in (a). (d) Inpp5e +/+ , Inpp5e +/‒ and Inpp5e ‒/‒ MEFs cultured with or without FBS were analyzed by Western blot with the indicated antibodies. Tubulin was used as loading control and phospho-retinoblastoma (pRb) as cell cycle marker. Note INPP5E-dependence of PDGFRα receptor levels in serum-starved MEFs.

    Article Snippet: Other reagents included GFP-Trap_MA beads (Proteintech, gtma), mouse anti-Flag M2 magnetic beads (Sigma, M8823), sodium orthovanadate (Alfa Aesar, J60191) and the following recombinant human growth factors: PDGF-AA (Proteintech, HZ-1215), TGF-β1 (R&D Systems, 240-B), Insulin (Gibco, 12585014), IGF-I (Proteintech, HZ-1322) and EGF (Proteintech, HZ-1326).

    Techniques: Western Blot, Control, Cell Culture, Marker